Enzymatic synthesis of polymers containing nicotinamide mononucleotide.

نویسندگان

  • R Liu
  • L E Orgel
چکیده

Nicotinamide mononucleoside 5'-diphosphate in its reduced form is an excellent substrate for polynucleotide phosphorylase from Micrococcus luteus both in de novo polymerization reactions and in primer extension reactions. The oxidized form of the diphosphate is a much less efficient substrate; it can be used to extend primers but does not oligomerize in the absence of a primer. The cyanide adduct of the oxidized substrate, like the reduced substrate, polymerizes efficiently. Loss of cyanide yields high molecular weight polymers of the oxidized form. Terminal transferase from calf thymus accepts nicotinamide mononucleoside 5'-triphosphate as a substrate and efficiently adds one residue to the 3'-end of an oligodeoxynucleotide. T4 polynucleotide kinase accepts oligomers of nicotinamide mononucleotide as substrates. However, RNA polymerases do not incorporate nicotinamide mononucleoside 5'-triphosphate into products on any of the templates that we used.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Enzymatic Synthesis of Triphosphopyridine Nucleotide

Von Euler and Vestin (1) demonstrated the synthesis of triphosphopyridine nucleotide (TPN) when diphosphopyridine nucleotide (DPN) was incubated with adenosine triphosphate (ATP) in the presence of crude yeast maceration juice. In subsequent reports (2) from the Stockholm laboratory, several mechanisms for this reaction were considered but none was established. With crude pigeon liver fractions...

متن کامل

Enzymatic Synthesis of Triphosphopyridine Nucleotide

Von Euler and Vestin (1) demonstrated the synthesis of triphosphopyridine nucleotide (TPN) when diphosphopyridine nucleotide (DPN) was incubated with adenosine triphosphate (ATP) in the presence of crude yeast maceration juice. In subsequent reports (2) from the Stockholm laboratory, several mechanisms for this reaction were considered but none was established. With crude pigeon liver fractions...

متن کامل

Preparation and properties of some nicotinamide adenine dinucleotide analogues with pentose and purine modifications.

The structure of nicotinamide adenine dinucleotide was proved conclusively by chemical synthesis (1) and isolation (2) of the biologically active coenzyme from a reaction in which the 5,5’dinucleoside pyrophosphate was formed by condensing together the two component 5’-mononucleotides, adenosine monophosphate and nicotinamide mononucleotide, with dicyclohexylcarbodiimide or trifluoroacetic acid...

متن کامل

Enzymatic Formation of Uridine Diphosphoglucuronic

Many studies have shown that, although glucose is converted to glucuronides without cleavage of the carbon chain (2--S), free glucuronic acid functions poorly, if at all, as a precursor of glucuronic acid-containing compounds in living systems (e.g. phenol or alcohol glucuronides, mucopolysaccharides) (9-l 1). An important advance in elucidating the nature of the intermediates in the biosynthes...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Nucleic acids research

دوره 23 18  شماره 

صفحات  -

تاریخ انتشار 1995